A/B Testing Email Campaigns: Beyond Open Rates

Why Most Email A/B Tests Fail to Deliver Real Results

Email remains one of the highest-ROI marketing channels available, returning an average of $42 for every $1 spent, according to the Data & Marketing Association. Yet many marketers squander this potential by focusing their A/B testing efforts on vanity metrics rather than business outcomes.

I’ve reviewed hundreds of email testing programs across industries, and the pattern is clear: marketers who test subject lines solely to increase open rates often see no meaningful improvement in their bottom line. The most successful email marketers test with revenue and conversions as their north star metrics.

Let’s explore how to transform your email A/B testing from a superficial exercise into a powerful driver of business results.

Comparison of email variations with key test elements highlighted

The Email Testing Hierarchy: What to Test for Maximum Impact

Not all email elements are created equal when it comes to testing potential. I’ve organized them into a hierarchy based on their typical impact on business results:

Tier 1: High-Impact Elements (Test These First)

  • Offer structure – The fundamental value proposition presented
  • Call-to-action (primary) – Both wording and design
  • Email timing/frequency – When and how often emails are sent
  • Audience segmentation – How recipients are grouped and targeted
  • Email content structure – Length, format, and organization

Tier 2: Medium-Impact Elements

  • Subject line – Opening hook (important but overemphasized by most marketers)
  • Preview text – The snippet visible in inboxes
  • Hero image/visual – Primary visual element
  • Personalization approach – How custom elements are incorporated
  • Social proof elements – Testimonials, reviews, usage statistics

Tier 3: Lower-Impact Elements (Test After Higher Tiers)

  • Secondary calls-to-action – Additional desired actions
  • Footer elements – Company info, unsubscribe options
  • Button colors/design – Visual aspects of CTAs
  • Body font/styling – Text appearance
  • Image-to-text ratio – Balance between visual and written content

This hierarchy isn’t absolute—for some businesses, certain elements may prove more impactful. However, it provides a strategic starting point that avoids the common trap of endless subject line testing with minimal business impact.

Beyond the Subject Line: High-Impact Tests to Transform Your Results

Let’s explore specific high-impact email A/B tests that have delivered significant results for real marketing programs:

Offer Structure Testing

Compare fundamentally different value propositions or offer frameworks:

Example Test: An e-commerce retailer tested:

  • Control: 20% off any purchase
  • Variation: $25 off purchases of $100 or more

Results: While the percentage discount drove 11% higher open rates (likely due to the subject line), the dollar amount threshold offer generated 37% higher revenue per email by encouraging larger cart sizes.

Email Content Structure Testing

Test different approaches to organizing your email content:

Example Test: A B2B software company tested:

  • Control: Traditional long-form email with multiple paragraphs, images, and calls-to-action
  • Variation: Ultra-short email that looked like a personal message from an account executive with just two sentences and one link

Results: The brief, personal-looking email drove 104% more demo bookings despite containing significantly less information and brand styling.

Audience Segmentation Testing

Test different ways of dividing your audience for targeted content:

Example Test: A travel company tested:

  • Control: Segmentation based on past purchase category (cruises, flights, hotels, etc.)
  • Variation: Segmentation based on identified trip motivation (luxury, budget, adventure, family, etc.)

Results: The motivation-based segmentation increased bookings by 41% compared to the product-category segmentation.

Frequency and Timing Testing

Test when and how often to send emails:

Example Test: A media subscription service tested:

  • Control: Promotional emails sent on Tuesdays and Thursdays at 10 AM
  • Variation: Testing revealed optimal times varied dramatically by customer segment, with working professionals engaging evenings 7-9 PM and retirees engaging mornings 7-9 AM

Results: Implementing segment-based timing increased overall conversion rate by 23% with no content changes.

Email marketing a/b testing

Structuring Email Tests for Valid Results

Email testing requires careful design to ensure your results are reliable and actionable:

Test One Variable at a Time

Unless you’re using multivariate testing with large list sizes, isolate variables to know exactly what drove results:

Poor Test: Different subject line, header image, and CTA button Good Test: Same email with only CTA button variations

Ensure Statistical Validity

Email list segments need to be large enough for meaningful results:

Baseline Click RateMinimum Sample Size Per Variation (For 95% Confidence)
1%15,200 recipients
3%5,000 recipients
5%3,000 recipients
10%1,500 recipients

For smaller lists, focus on larger potential improvements and accept lower confidence levels or longer testing periods.

Control External Variables

To prevent skewed results:

  • Send test variations at the same time
  • Ensure random distribution between segments
  • Maintain consistent tracking parameters
  • Monitor for deliverability differences between variations

Test Across Multiple Sends

Avoid drawing conclusions from a single test:

  • Confirm findings across 3-5 campaigns minimum
  • Test with different audience segments
  • Validate across various offer types
  • Check if results hold during different seasons/times

A retail client jumped to implement a new content approach after a successful test, only to discover in subsequent testing that their initial results were an outlier. Establishing testing patterns across multiple campaigns would have prevented this costly mistake.

The Four Email Metrics That Actually Matter

Move beyond open rates to metrics that drive business impact:

1. Conversion Rate

The percentage of email recipients who completed your desired action (purchase, signup, etc.). This is the most direct measurement of email effectiveness.

2. Revenue Per Email Sent

Total revenue generated divided by number of emails delivered. This captures both conversion rate and order value impacts.

3. Click-to-Conversion Rate

The percentage of people who click through and then convert. This isolates the effectiveness of your post-click experience.

4. Subscriber Lifetime Value

How email engagement affects long-term customer value and retention. The most strategic metric but requires longer measurement periods.

Secondary metrics like open rate and click rate still matter as diagnostic tools, but they shouldn’t be your primary success metrics.

Building an Email Testing Roadmap: From Quick Wins to Strategic Testing

Instead of random testing, develop a structured email testing plan:

Phase 1: Foundational Testing (1-2 Months)

Focus on establishing baselines and finding major improvement opportunities:

  • Optimal sending time by segment
  • Basic offer structure testing
  • Primary CTA optimization
  • Content format preferences

Phase 2: Segmentation Testing (2-3 Months)

Test different ways to divide your audience for maximum relevance:

  • Behavioral segmentation tests
  • Demographic segmentation tests
  • Engagement-based segmentation tests
  • Purchase history segmentation tests

Phase 3: Personalization and Advanced Testing (Ongoing)

Refine your approach with more sophisticated testing:

  • Dynamic content element testing
  • Personalization algorithm testing
  • Behavioral trigger optimization
  • Predictive content testing

For a financial services client, this structured approach increased email-driven revenue by 142% over nine months, compared to the minimal gains they saw from their previous random testing approach.

Email A/B Testing Tools: From Basic to Advanced

The right tools can dramatically improve your testing capabilities:

Basic Tools (Getting Started)

  • Native ESP testing features (MailChimp, Constant Contact, etc.)
  • Google Analytics for conversion tracking
  • Basic heat mapping for click analysis

Intermediate Tools (Expanding Capabilities)

  • Dedicated testing platforms (Optimizely, VWO)
  • Customer journey analytics
  • Advanced ESPs with automation (HubSpot, Klaviyo)

Advanced Tools (Sophisticated Programs)

  • AI-powered testing and optimization
  • Predictive analytics platforms
  • Custom data warehouse integration
  • Multi-channel testing coordination

The tools you choose should align with your testing maturity and list size. A mid-sized B2B company I worked with wasted thousands on advanced tools when they lacked the list size to utilize the capabilities effectively.

Common Email Testing Mistakes and How to Avoid Them

Learn from these frequently observed testing errors:

Mistake 1: Testing Too Many Elements Simultaneously

Problem: Unable to determine which change drove results Solution: Use a controlled testing approach with isolated variables

Mistake 2: Optimizing for Opens Instead of Conversions

Problem: Improved vanity metrics but no business impact Solution: Always include conversion tracking in your success metrics

Mistake 3: Insufficient Sample Sizes

Problem: Drawing conclusions from statistically invalid results Solution: Use sample size calculators and extend testing duration for smaller lists

Mistake 4: Ignoring Mobile Experience

Problem: Tests that work on desktop fail on mobile, where 60%+ of emails are opened Solution: Always preview and test both mobile and desktop versions

Mistake 5: Failure to Document and Scale Learnings

Problem: Same tests repeated, successful tactics not implemented widely Solution: Maintain a testing repository and systematic implementation process

An agency I consulted with had run over 150 email tests for clients but had no centralized system for tracking results. This meant they frequently repeated tests and failed to apply learnings across accounts—a massive efficiency opportunity we corrected with a simple testing database.

From Email Testing to Omnichannel Optimization

The most sophisticated marketers coordinate email testing with other channel testing:

  • Test messaging consistency across email, ads, and landing pages
  • Coordinate offers between email and retargeting campaigns
  • Apply email content insights to social media strategies
  • Use email engagement data to inform website personalization

This integrated approach creates a multiplier effect where insights from one channel improve performance across your entire marketing ecosystem.

Want to explore other aspects of A/B testing? Check out our Ultimate Guide to A/B Testing for a comprehensive overview, or learn about Finding Statistical Significance in A/B Tests to ensure your results are reliable.

Remember, effective email testing isn’t about tweaking subject lines for marginally better open rates—it’s about discovering what truly resonates with your audience and drives them to action. With the strategic approach outlined here, you can transform your email program from a basic communication channel into a conversion and revenue powerhouse.